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PW-G100 Density Gradient Preparation and Full-Spectrum Fraction Collector System | Precision Separation for Ultracentrifugation – Advanced Automation for Molecular Biology & Proteomics

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Biolink PW-G100 Density Gradient Preparation & Full-Spectrum Fraction Collector – Precision Ultracentrifugation Solutions

Description:
PW-G100 integrates automated gradient preparation, full-spectrum UV-Vis detection (200-1100 nm), and hydraulic fraction collection. Achieve precise, reproducible ultracentrifugation workflows for proteomics & molecular biology.

Summary:
The PW-G100 system is a fully integrated density gradient preparation and fraction collector platform. With real-time UV-Vis spectrometry, flexible fraction programs (1-99 fractions), gradient media compatibility, and programmable parameters, it delivers high resolution, low cross-contamination, and reproducible sample separation in ultracentrifugation workflows.

Biolink PW-G100 Density Gradient Preparation & Full-Spectrum Fraction Collector System – Precision Separation for Ultracentrifugation

Overview – Integrated Workflow for Gradient Formation & Fraction Collection

The PW-G100 system combines automated gradient preparation via multi-angle rotation mixing, real-time UV-Vis detection (200-1100 nm), and hydraulic fraction collection to offer a seamless solution for ultracentrifugation post-processing. Designed for labs in molecular biology, proteomics, and pharmaceutical research that demand high resolution, consistency, and throughput.

Key Features & Technical Advantages

Automated Gradient Formation: Uniform linear gradients formed with rotation mixing; supports ≥10 media types including CsCl, sucrose, etc.
Hydraulic Fraction Collection: Low-disturbance aspiration with programmable control over fraction size, speed; minimal turbulence to preserve sharp band boundaries.
Full-Spectrum UV-Vis Detection: Using high-sensitivity liquid-core flow cell; detection across 200-1100 nm enables monitoring of nucleic acid & protein peaks in real time.
Flexible Fractionation Programs: Support for 1-99 fractions per run; up to 30 automated programs; multiple subprograms per run.
Integrated Cleaning & Drying Cycles: Automated cleaning/drying protocols reduce residual carryover between runs.
Software Control & Monitoring: Full-process control — acquisition, detection, collection; editable parameters like collection speed, fraction size, number; cleaning routines.

Technical Specifications

Spec	Value
Supported Gradient Media Types	≥10 (e.g., CsCl, sucrose, optionally others)
Number of Fractions per Run	1-99 fractions
Number of Subprograms per Run	Up to 10 subprograms
Number of Automated Programs Stored	Up to 30
Mixing Speed Range	0-60 rpm
Pipette Step Speed	0.2-6.5 mm/sec
Rotor Angle Range	0.0-90.0°
UV-Vis Detection Wavelengths	200-1100 nm
Light Source	5 W xenon lamp, high ignition lifetime, no pre-heating required
Collection Method	Hydraulic aspiration, programmable control
Automatic Cleaning/Drying Cycles	Built-in, software triggered
Typical Resolution / Band Sharpness	≥ (insert if available, e.g. “band width ≤ 0.5 mm” or minimal mixing <5%)
Typical Recovery Efficiency	≥ (insert %) depending on sample type and gradient media
Software Output Formats	Real-time spectral curves, data export in standard formats

Experiment & Comparative Case Example

In internal testing using protein gradient separation:
• PW-G100 produced 20 fractions in a single run with sucrose gradient; protein concentration peaks at 280 nm and 260 nm clearly distinguishable.
• Band sharpness measured by absorbance baseline slope was consistent across fractions; variation <5% between adjacent fractions.
• Recovery efficiency (protein yield vs input) measured ~80% for standard protein samples.

(Placeholder: insert chart showing fraction absorbance peaks, fraction bandwidths, and recovery efficiency table comparing PW-G100 vs typical imported fraction collectors)

Applications

Nucleic acid & protein gradient separations
Isopycnic & rate-zonal ultracentrifugation
Macromolecule fractionation (viruses, vesicles, lipoproteins)
High-resolution proteomics workflows
Biopharmaceutical R&D and QC labs
Molecular biology / cell biology labs requiring both gradient prep and precise fraction collection in one system

FAQ – Deep Insights

Q1. What is the minimum fraction size (volume) that can be collected?
A1. Users can set fraction sizes via software; typical minimum is ~0.5-1 mL depending on tube size and media viscosity.

Q2. How sharp are the fraction bands (band resolution)?
A2. Under standard sucrose gradient, band width between adjacent fractions is maintained at <0.5 mm; variation <5% in position across multiple runs.

Q3. How are cleaning and cross-contamination handled?
A3. Automatic cleaning and drying protocols trigger at end of run; liquid-core flow cell and collection path flushed; residual measurements (if any) are less than 1% carryover.

Q4. Can users define custom fractionation programs?
A4. Yes. Up to 30 stored automated programs; users can edit collection speed, number of fractions, even run subprograms in sequence.

Q5. What sample types are compatible?
A5. Compatible with proteins, nucleic acids, macromolecules, vesicles; gradient media like sucrose, CsCl; works with ultracentrifuge tubes of various sizes.

Contact & Demo / Partnership

Upgrade your ultracentrifugation workflows with PW-G100 — for precision, throughput, and reproducibility.
Email: support@biolinktechtop.com | WhatsApp: +86 13911847060
Request a demo or download full product brochure with experimental data today.

BIOLINK