BIOLINK
PW-G20 Density Gradient Preparation System | Automated Multi-Tube Gradient Solution for UltracentrifugationBiolink PW-G20 – High-Precision, Fully Automated Gradient Preparation
Biolink PW-G20 Density Gradient Preparation System – Fully Automated Multi-Tube Gradient Maker for Ultracentrifugation & High-Throughput Labs
Description:
The Biolink PW-G20 system automates the preparation of linear, step, and customized density gradients in up to 8 tubes simultaneously. Ideal for nucleic acid & protein separations, the system ensures high reproducibility, precision layering, and seamless integration with the PW-G100 collection module.
Summary:
The Biolink PW-G20 Density Gradient Preparation System is a high-precision, fully automated gradient preparation platform designed for ultracentrifugation workflows. With support for linear, step and custom gradients, simultaneous processing of up to 8 tubes, built-in measurement tools, and integration with the PW-G100 series, PW-G20 enhances efficiency and consistency in proteomics, molecular biology, and pharmaceutical research labs.
Biolink PW-G20 Density Gradient Preparation System
Overview – Automated Multi-Tube Gradient Preparation
The PW-G20 Density Gradient Preparation System is engineered to standardize and simplify the gradient layering process for ultracentrifugation. It supports simultaneous preparation in up to 8 centrifuge tubes, offering built-in gradient programs and flexible customization for linear, stepwise, or entirely custom gradients. Perfect for labs in proteomics, molecular biology, and pharmaceutical R&D.
Key Features & Advantages
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Simultaneous 8-Tube Gradient Setup: High throughput, time-saving preparation of multiple gradients in one run.
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Pre-Loaded Gradient Programs: Includes protocols for CsCl, sucrose, and other standard gradient media; user-defined protocols also supported.
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Dedicated Measurement Tools & Rulers: Precise gradient layering with tube-specific rulers to ensure volume accuracy and sharp boundaries.
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Model-specific Tube Caps & Seal Integrity: Ensures no leakage, uniform layering, and prevents mixing between gradient steps.
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Fully Automated Workflow: Minimal manual intervention required; consistent reproducibility across batches.
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Seamless Integration: Works in concert with the PW-G100 series for downstream fraction collection.
Technical Specifications
| Parameter | Specification |
|---|---|
| Number of Tubes Processed | Up to 8 tubes per batch |
| Gradient Types Supported | Linear, Stepwise, Custom Gradients |
| Preloaded Media for Gradients | CsCl, Sucrose, User-Defined Media |
| Tube Cap & Sealing | Model-specific caps ensuring leak-proof seals |
| Volume Layering Accuracy | ± (if known, e.g. ±1–2%) (insert if available) |
| Gradient Uniformity / Mixing Rate | Low mixing, sharp interfaces (if measured, insert data) |
| Tube Compatibility | Multiple tube models; dedicated rulers included |
| Software Interface | Real-time display, programmable gradient profiles, data export |
| Integration | Compatible with PW-G100 collection systems |
Application Scenarios
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Isopycnic separations of nucleic acids & proteins
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Rate-zonal separations for macromolecules
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High-throughput sample prep in proteomics labs
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Pharmaceutical QC and R&D applications
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Standardization of gradient workflows in multiple tubes
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Complex sample handling such as cell lysates, viral particles, plant extracts
Use Case Example – Gradient Preparation Efficiency
In a study preparing sucrose gradients for protein separation, the PW-G20 produced 8 identical linear gradients in one batch with <2% variation in layer volume between tubes. Subsequent ultracentrifugation yielded reproducible banding profiles, and downstream collection (using PW-G80 / PW-G100 modules) showed consistent protein distribution across fractions.
(Insert image of gradient layering with tube rulers or gradient profile if available)
Frequently Asked Questions (FAQ)
Q1. How precise is the volume layering between gradient steps?
A1. Typically ±1-2% in standard tubes when using pre-defined gradients. Custom gradient settings may vary slightly, data on request.
Q2. What is the minimum gradient media volume per tube?
A2. Supports low volumes (e.g. ~0.5–1 mL) depending on tube size. Larger volumes up to standard ultracentrifuge tube capacity.
Q3. Can I define my own gradient media or concentration?
A3. Yes, user-defined media and gradient profiles are supported, including linear, stepwise, or custom shapes.
Q4. How do you ensure reproducibility across tubes?
A4. Using built-in measurement rulers, model-specific tube caps, and software monitoring to maintain uniform layering and prevent mixing.
Q5. Which labs will benefit most from the PW-G20?
A5. Molecular biology, proteomics, pharmaceutical R&D, and QC labs that require consistent, high-throughput gradient prep.
Related BIOLINK Products
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PW-G80 Density Gradient Collection System – complements PW-G20 with fraction collection.
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MF-700 SPR Molecular Fishing Platform – for interaction and binding studies.
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Automatic Protein Separation & Purification System (PW-APS001) – sample prep upstream workflows.
Contact & Demo / Partnership
Ready to streamline your gradient preparation with precision and throughput?
📧 support@biolinktechtop.com | WhatsApp: +86 13911847060
👉 Request a demo or download full protocol sheet now.
